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Sometimes you ll find yourself in the finger-pointing circle game, where the hardware vendor blames the OS vendor, who blames the storage vendor, who blames the application vendor, who blames the hardware vendor again All the while, of course, your system is down If the failed server is a critical one, this sort of hours- or days-long outage due to vendor bickering is simply unacceptable What can you do You could take your applications off the Unix or Windows server you ve installed them on and put them on a multimillion-dollar fault-tolerant server instead Unfortunately, a fault-tolerant (FT) server, which is designed with redundant hardware (often triple-redundant hardware; there is at least one quad-redundant system on the market) so that if one component fails others can instantly step in and take over for them, may still not offer adequate protection.

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Although the FT vendors may make enhancements to their drivers and operating system, FT systems are no less vulnerable to software issues than more conventional systems What s more, by their nature, FT systems are closed systems that do not offer the flexibility or connectivity of conventional systems, because those benefits can introduce risk to the system It is difficult to migrate existing applications to FT systems, because they are not always compatible with conventional systems FT systems are popular in certain high-end applications, such as gaming (casinos and lotteries), and air traffic control, where the benefits that they provide offset their cost A more practical and less expensive solution is to take two or more conventional servers and connect them together with some controlling software, so that if one server fails, the other server can take over automatically.

See Ref. 11. [Reprinted, by permission, from B. Danielsson and F. Winquist in Biosensors: A Practical Approach , A. E. G. Cass, Ed., Oxford University Press, New York, 1990. The Practical Approach Series, Edited by D. Rickwood and B. D. James. # Oxford University Press 1990.]

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The takeover occurs with some interruption in service, but that interruption is usually limited to just a few minutes To ensure data consistency and rapid recovery, the servers should be connected to the same shared disks The discussions in this chapter assume that the servers are located within the same site, and generally in the same room Migrating critical applications to a remote site is a disaster recovery issue, and while it seems similar to the local case, it actually introduces many new.

reactions are accompanied by signi cant enthalpy changes, thermistors with immobilized enzymes can detect local temperature changes in the environment near the thermistor surface. For example, the reactions catalyzed by catalase, GO and NADH dehydrogenase are accompanied by enthalpy changes of 100, 80, and 225 kJ/mol, respectively. Figure 7.10 shows a schematic diagram of an enzyme thermistor used to test a variety of immobilized enzymes in a ow-through biosensing device. The temperature probe is sealed inside a ow-through reaction chamber, which is encased in an aluminum constant-temperature jacket (to avoid responses due to ambient temperature uctuations). The Wheatstone bridge allow differential measurement of both termistors, thus permitting measurements of small temperature changes (down to 10 4 C). By using devices similar to that shown in Figure 7.10, many immobilized enzymes have been tested. Table 7.1 summarizes the analytes, the enzymes, and the dynamic ranges over which the enzyme thermistors provide substrate quantitation. 7.4. EVALUATION OF BIOSENSOR PERFORMANCE In addition to the usual evaluation parameters for analytical methods ( 16), the sensitivity, detection limit, dynamic range, and precision pro le, biosensors are also characterized with respect to the rapidity of their response and recovery. This

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